CT value depends on the number of series of cycles after the virus is detected.
In order to standardize qPCR nomenclature, the MIQE minimum information for publication of quantitative real-time PCR experiments guidelines recommend using C q value.
The threshold level and C q value on a real-time PCR amplification curve.
Other normalization methods include the Dealt-Cq Method and the Pfaffl Method.
We consulted the aforementioned radiologist at Tongji Hospital regarding these two concerns.
The major concerns are summarized as follows.
Amplifications take different series of cycles.
COVID-CT The utility of this dataset has been confirmed by a senior radiologist in Tongji Hospital, Wuhan, China, who has performed diagnosis and treatment of a large number of COVID-19 patients during the outbreak of this disease between January and April.
It is wise to use at least two reference genes, and bear in mind that what may be a reference gene for one study may not be suitable for another.
CT value indicates a virus present in the throat, not in the lungs.
Threshold (CT) values from COVID-19 PCR tests. 1. What is a CT value? CT stands for “Cycle Threshold” and indicates how many times a machine needed to try to copy a particular virus’s genetic material before being able to detect that material on a particular test called a Polymerase Chain Reaction (PCR) test. The CT value can be
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