Background Early and accurate identification of infection viruses among children can benefit the personalized medical treatment and management, and reduce the future occurrence of serious symptoms. Thus, it is critical to develop a high-throughput multiplex real-time RT-PCR method to improve the accuracy and efficiency in routine clinical lab tests. Methods We developed a real time RT-PCR.
The sequin (zecchino) is a gold coin consisting of 3.5 grams (0.12 oz) of .986 gold, minted by the Republic of Venice.The design of the coin remained relatively unchanged for more than 500 years, from its introduction in 1284 to the fall of the Venetian Republic at the hands of Napoleon on 12 May 1797.
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