An additional 5 eggs were inoculated in a similar manner with the original culture medium.
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Although the mutant is very attenuated in young infants, it nonetheless stimulates production of protective levels of serum PIV3 antibodies.
Mycoplasma Sterility: The assay for mycoplasma was performed using both the direct Broth Enrichment and Agar Procedures and the indirect Indicator Cell Culture Procedure with the AGMK cell suspension Passage 17A, Lot 4706 containing approximately 5Ã—10 5 cells per ml.
In addition, the invention provides a method for recovering and serially propagating specific viruses in the cell line that subsequently can be used for production of a viral vaccine.
To achieve these and other advantages and in accordance with the purpose of the invention, as embodied and broadly described, the invention provides a cell line derived from the paired kidneys of an African green monkey, which is free of demonstrable adventitious microbial agents.
In contrast to MA104 or primary monkey kidney cells, cell substrates certified for vaccine production, such as simian FRhL-2 or Vero cells, either fail to support growth of completely homologous hurn rotaviruses or allow only poor growth.
African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses. The preparation of live, attenuated human influenza virus vaccines and of large quantities of inactivated vaccines after the emergence or reemergence of a pandemic influenza virus will require an alternative host cell system, because embryonated chicken eggs will likely be insufficient and suboptima ….
The genome landscape of the african green monkey kidney-derived vero cell line Continuous cell lines that originate from mammalian tissues serve as not only invaluable tools for life sciences, but also important animal cell substrates for the production of various types of biological pharmaceuticals.
DNA – Vero African Green Monkey Kidney Cells African Green Monkey kidney cells are called VERO cells and are used in vaccines. They were established from the kidney epithelial cells extracted from a normal adult African Green monkey. Vero cells are a lineage of cells used in cell cultures and are.
Regardless, HEp-2/HeLa cells remain a transformed, human, epithelial cell line. In contrast, Vero cells were generated through the sequential passage of adult African green monkey kidney cells (Rhim et al., 1969), and they are a nontransformed, primate, fibroblast cell line. This raises the possibility that differences in species, tissue origin.
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