Procedure of Indole Test Take a sterilized test tubes containing 4 ml of tryptophan broth. Inoculate the tube aseptically by taking the growth from 18 to 24 hrs culture. Incubate the tube at 37°C for 24-28 hours. Add 0.5 ml of Kovac’s reagent to the broth culture. Observe for the presence or absence of ring. Indole Spot Reagent (DMACA) Procedure
Indole test is the a biochemical test performed on bacterial species to detect the ability of an organism to degrade the amino acid tryptophan and produce indole. It is used as the part of the IMViC tests, a set of four useful reactions that are commonly designed for the differentiation of enterics (members of family Enterobacteriaceae).
Indole positive and negative test 2- Spot test Place several drops (1 -1.5 ml) of Indole Spot reagent (1% or 5% p-methylaminobenzaldehyde or 1% p-dimethylaminocinnamaledhyde)) on a piece of filter paper. Spread an isolated pure colony (from an 18-24 hour culture) on the saturated surface of the filter paper using a sterile loop Examine immediately
The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to convert tryptophan into indole. This division is performed by a chain of a number of different intracellular enzymes, a system generally referred to as “tryptophanase.”
Indole test is one of the ideal methods used to distinguish the E.coli from Enterobacter and Keibsiella. The method distinguishes and characterizes the bacteria based on the ability of bacteria to degrade the tryptophan (an amino acid) into indole, and hence the name Indole test. Not all the coliforms have the ability to degrade tryptophan, and.
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